Sequential Bioprocess with Gluconobacter oxydans and Candida tropicalis for Gluconic Acid and Single-Cell Protein Production from Enzymatic Hydrolysate

To meet the growing global demand for gluconic acid as a cement and concrete retarder, inexpensive abundant lignocellulosic materials are regarded most suitable alternatives to starchy materials. However, their enzymatic hydrolysate contains not only glucose but also xylose, which negatively affects performance of retarder. Notably, is preferentially bio-oxidized into by Gluconobacter oxydans, cannot be metabolized Candida tropicalis. Given this, an artificially designed biological cascade process, respectively employing oxydans tropicalis, was established successfully carry out conversion acid, xylose single-cell protein, using corncobs feedstock. This sequential fermentation process produced 95.8 g/L 9.0 protein from one liter that initially contained 98.1 25.4 xylose. The mass-balance calculation showed approximately 280 grams 27 could harvested 1000 corncob results suggest above-mentioned two-step bioconversion method efficient in utilizing hydrolysates.